On-line video abstraction

Tesis doctoral inédita. Universidad Autónoma de Madrid, Escuela Politécnica Superior, abril de 201

Extensibility of adaptation capabilities in the CAIN content adaptation engine

Also published online by CEUR Workshop Proceedings (CEUR-WS.org, ISSN 1613-0073)This paper describes the extensibility mechanism that has been incorporated to the CAIN Adaptation Engine, that provides audiovisual content adaptation based on user preferences, network capabilities and terminal limitations. The integration of new adaptation modules needs no code modifications in the core system, so it does not have to be recompiled for adding or modifying adaptation modules.This work is partially supported by the European Commission 6th Framework Program under project FP6-001765 (aceMedia). This work is also supported by the Ministerio de Ciencia y Tecnología of the Spanish Government under project TIN2004-07860 (MEDUSA) and by the Comunidad de Madrid under project P-TIC-0223-0505 (PROMULTIDIS). The authors want to thank Víctor Fernández-Carbajales for successful testing of the extensibility mechanism

Hidroforraje de Leucaena leucocephala para alimentar conejos

Leucaena leucocephala hydroforage nutritional value for rabbit feeding was found out by determining its chemical composition, amino-acid profile, antinutritional factors presence, physical properties, and in vitro digestibility using a completely randomized design. Results showed 23 % of dry matter, high levels of raw protein (27,2 %), 31,38 % of neutral detergent fiber, 19,06 % of acid detergent fiber, and 8,43 % of ashes. Proper levels of essential aminoacids for rabbits, i.e., lycine, threonine, phenylalanine, and leucine were found out; however, methionine levels were low. Antinutritional factors decreased by the hydroforage aging process: tannin concentration in L. leucocephala seeds dropped from 9 % to 1,1 % following 20 days of hydroforage aging. Similarly, 4,5 % of mimosine was also detected in L. leucocephala seeds, but none appeared after a 25-day aging process of the hydroforage. Fiber solubility was 26,61 % with a water absorption capacity of 11,98 (gg-1 FDN). Dry matter digestibility was 67,73 %. These features fit feeding requirements for rabbits.Con el objetivo de determinar el valor nutritivo del hidroforraje de Leucaena leucocephala (HFL) para la alimentación de conejos, se determinó: la composición química, perfil aminocídico, presencia de factores antinutricionales, propiedades físicas y digestibilidad in vitro. Se empleó un diseño completamente aleatorio. El alimento mostró 23 % de materia seca, altos niveles de proteína bruta (27,2 %), 31,18 % de fibra neutro detergente, 19,06 % de fibra ácido detergente y 8,43 % tenores de ceniza. Este alimento tiene adecuados niveles de lisina, treonina, fenilalanina y leucina, aminoácidos esenciales para los conejos; en cambio los niveles de metionina fueron bajos. Se encontró que a medida que aumentaba la edad del hidroforraje, disminuían los factores antinutricionales: los taninos se presentaron en las semillas de leucaena en 9 %, y a los 20 días del alimento su concentración era de 1,1 %. Se detectó presencia de mimosina en las semillas de esta planta (4,5 %); no así a los 25 días del hidroforraje. La solubilidad de fibra fue 26,61 % con capacidad de absorción de agua de 11,98 (gg-1FDN). La digestibilidad de la materia seca fue 67,73 %. Estas características se ajustan a las exigencias alimenticias de la especie cunícola

Antimicrobial Activity of Biogenic Silver Nanoparticles from Syzygium aromaticum against the Five Most Common Microorganisms in the Oral Cavity

ARTICULO DE ACCESO LIBREAbstract: Syzygium aromaticum (clove) has been used as a dental analgesic, an anesthetic, and a bioreducing and capping agent in the formation of metallic nanoparticles. The main objective of this study was to evaluate the antimicrobial effect in oral microorganisms of biogenic silver nanoparticles (AgNPs) formed with aqueous extract of clove through an ecofriendly method “green synthesis”. The obtained AgNPs were characterized by UV-Vis (ultraviolet-visible spectroscopy), SEM-EDS (scanning electron microscopy–energy dispersive X-ray spectroscopy), TEM (transmission electron microscopy), and ζ potential, while its antimicrobial effect was corroborated against oral Grampositive and Gram-negative microorganisms, as well as yeast that is commonly present in the oral cavity. The AgNPs showed absorption at 400–500 nm in the UV-Vis spectrum, had an average size of 4–16 nm as observed by the high-resolution transmission electron microscopy (HR-TEM), and were of a crystalline nature and quasi-spherical form. The antimicrobial susceptibility test showed inhibition zones of 2–4 mm in diameter. Our results suggest that AgNPs synthesized with clove can be used as effective growth inhibitors in several oral microorganisms.S/

Pedagogía Ignaciana 4. Reflexiones a diez años de las Características de la educación de la Compañía de Jesús

Obra que reúne las ponencias presentadas en el Seminario Internacional realizado en el Instituto Tecnológico y de Estudios Superiores de Occidente, del 5 al 8 de diciembre de 1996, con motivo de la celebración del décimo aniversario de haberse publicado las Características de la educación de la Compañía de Jesús. Allí los ponentes reflexionaron sobre el contexto de la educación jesuítica, su propuesta educativa y puesta en práctica, y sus retos y desafíos.ITESO, A.C

Mechanical Properties and Antibacterial Effect on Mono-Strain of Streptococcus mutans of Orthodontic Cements Reinforced with Chlorhexidine-Modified Nanotubes

Recently, several studies have introduced nanotechnology into the area of dental materials with the aim of improving their properties. The objective of this study is to determine the antibacterial and mechanical properties of type I glass ionomers reinforced with halloysite nanotubes modified with 2% chlorhexidine at concentrations of 5% and 10% relative to the total weight of the powder used to construct each sample. Regarding antibacterial effect, 200 samples were established and distributed into four experimental groups and six control groups (4 +ve and 2 −ve), with 20 samples each. The mechanical properties were evaluated in 270 samples, assessing microhardness (30 samples), compressive strength (120 samples), and setting time (120 samples). The groups were characterized by scanning electron microscopy and Fourier transform infrared spectroscopy, and the antibacterial activity of the ionomers was evaluated on Streptococcus mutans for 24 h. The control and positive control groups showed no antibacterial effect, while the experimental group with 5% concentration showed a zone of growth inhibition between 11.35 mm and 11.45 mm, and the group with 10% concentration showed a zone of growth inhibition between 12.50 mm and 13.20 mm. Statistical differences were observed between the experimental groups with 5% and 10% nanotubes. Regarding the mechanical properties, microhardness, and setting time, no statistical difference was found when compared with control groups, while compressive strength showed higher significant values, with ionomers modified with 10% concentration of nanotubes resulting in better compressive strength values. The incorporation of nanotubes at concentrations of 5% and 10% effectively inhibited the presence of S. mutans, particularly when the dose–response relationship was taken into account, with the advantage of maintaining and improving their mechanical properties

Breve historia de la Facultad de Odontología

La Facultad de Odontología se fundó el 27 de diciembre de 1963 e inició actividades el 3 de marzo de 1964. Años más tarde, el 19 de mayo de 1986, el H. Consejo Universitario aprobó su cambio de escuela a Facultad de Odontología. La conmemoración de 55 años de esta Facultad y 40 años de hermandad con las Universidades de Meikai y Asahi, se constituye un momento oportuno para manifestar el testimonio de cuanto han realizado hombres y mujeres que han dejado huella en esta institución

Empleo de Sistemas de Inmersión Temporal para la multiplicación de segmentos nodales de Dioscorea alata L. en el clon ‘Pacala Duclos’

In order to develop efficient propagation protocols, temporary immersion systems composed by two glass flask with 5 000 ml of capacity were used to multiply nodals segments from yam clone ‘‘Pacala Duclos’’. The working objectives for evaluation were: time and frequency of immersion, inoculum density, time and renewal volume of culture medium and multiplication stage length on propagation coefficient of nodal segments. Results permitted to define that using 10 minutes immersion time and immersion frequency each three and six hours, the highest increments in the multiplication coefficients (10.1 and 9.8 respectively, without significant differences between them) were obtained. The most favorable result for the multiplication coefficient (10.8) was obtained when 50 explants per culture flask was inoculated. When the renewal was carried out 24 days after culture with 2 000 ml culture medium, the most advantageous result was shown for the multiplication coefficient, with 14.1. Subcultures were developed 49 days after culture because the highest coefficient multiplication (15.2) was noticed. A higher culture time did not influence in the multiplication coefficient significantly.Key words: culture medium, Dioscorea alata L., inoculum density, multiplication coefficientCon el propósito de desarrollar protocolos de propagación eficientes se emplearon para la multiplicación de segmentos nodales en el clon de ñame ‘‘Pacala Duclos’’ sistemas de inmersión temporal conformados por dos frascos de vidrio de 5 000 ml de capacidad. Se definieron como objetivos de trabajo: evaluar el tiempo y la frecuencia de inmersión, densidad de inóculo, tiempo y volumen de renovación del medio de cultivo y la duración de la fase de multiplicación sobre el coeficiente de multiplicación de los segmentos nodales. Los resultados permitieron definir que con el empleo de 10 minutos de inmersión y frecuencias de inmersión cada tres y seis horas, se alcanzaron los mayores incrementos en los coeficientes de multiplicación (10.1 y 9.8 respectivamente, sin diferencias significativas entre ellos). Con una densidad de 50 explantes por frasco de cultivo se obtuvieron los mejores resultados para el coeficiente de multiplicación (10.8). Cuando la renovación se realizó a los 24 días de cultivo con un volumen de 2 000 ml del medio de cultivo el coeficiente se incrementó a 14.1. Se determinó realizar el subcultivo a los 49 días de cultivo, pues se obtuvo el más alto coeficiente de multiplicación de 15.2. Un tiempo de cultivo mayor no influyó de forma significativa en el coeficiente de multiplicación.Palabras clave: coeficiente de multiplicación, densidad de inóculo, Dioscorea alata L., medio de cultiv

Telomeric Repeat-Binding Factor Homologs in Entamoeba histolytica: New Clues for Telomeric Research

Telomeric Repeat Binding Factors (TRFs) are architectural nuclear proteins with critical roles in telomere-length regulation, chromosome end protection and, fusion prevention, DNA damage detection, and senescence regulation. Entamoeba histolytica, the parasite responsible of human amoebiasis, harbors three homologs of human TRFs, based on sequence similarities to their Myb DNA binding domain. These proteins were dubbed EhTRF-like I, II and III. In this work, we revealed that EhTRF-like I and II share similarity with human TRF1, while EhTRF-like III shares similarity with human TRF2 by in silico approach. The analysis of ehtrf-like genes showed they are expressed differentially under basal culture conditions. We also studied the cellular localization of EhTRF-like I and III proteins using subcellular fractionation and western blot assays. EhTRF-like I and III proteins were enriched in the nuclear fraction, but they were also present in the cytoplasm. Indirect immunofluorescence showed that these proteins were located at the nuclear periphery co-localizing with Lamin B1 and trimethylated H4K20, which is a characteristic mark of heterochromatic regions and telomeres. We found by transmission electron microscopy that EhTRF-like III was located in regions of more condensed chromatin. Finally, EMSA assays showed that EhTRF-like III forms specific DNA-protein complexes with telomeric related sequences. Our data suggested that EhTRF-like proteins play a role in the maintenance of the chromosome ends in this parasite

Staging Parkinson’s Disease Combining Motor and Nonmotor Symptoms Correlates with Disability and Quality of Life

COPPADIS Study Group.[Introduction] In a degenerative disorder such as Parkinson’s disease (PD), it is important to establish clinical stages that allow to know the course of the disease. Our aim was to analyze whether a scale combining Hoehn and Yahr’s motor stage (H&Y) and the nonmotor symptoms burden (NMSB) (assessed by the nonmotor symptoms scale (NMSS)) provides information about the disability and the patient’s quality of life (QoL) with regard to a defined clinical stage.[Materials and Methods] Cross-sectional study in which 603 PD patients from the COPPADIS cohort were classified according to H&Y (1, stage I; 2, stage II; 3, stage III; 4, stage IV/V) and NMSB (A: NMSS = 0–20; B: NMSS = 21–40; C: NMSS = 41–70; D: NMSS ≥ 71) in 16 stages (HY.NMSB, from 1A to 4D). QoL was assessed with the PDQ-39SI, PQ-10, and EUROHIS-QOL8 and disability with the Schwab&England ADL (Activities of Daily Living) scale.[Results] A worse QoL and greater disability were observed at a higher stage of H&Y and NMSB (). Combining both (HY.NMSB), patients in stages 1C and 1D and 2C and 2D had significantly worse QoL and/or less autonomy for ADL than those in stages 2A and 2B and 3A and 3B, respectively (; e.g., PDQ-39SI in 1D [n = 15] vs 2A [n = 101]: 28.6 ± 17.1 vs 7.9 ± 5.8; ).[Conclusion] The HY.NMSB scale is simple and reflects the degree of patient involvement more accurately than the H&Y. Patients with a lower H&Y stage may be more affected if they have a greater NMS burden.Peer reviewe